Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus


 A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5–5) was more conducive to somatic embryogenesis than a… (More)
DOI: 10.1007/s002990000268


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