Direct single stranded sequencing from agarose of polymerase chain reaction products.

@article{Green1990DirectSS,
  title={Direct single stranded sequencing from agarose of polymerase chain reaction products.},
  author={Andrew J. Green and A. Roopra and M. Vaudin},
  journal={Nucleic acids research},
  year={1990},
  volume={18 20},
  pages={6163-4}
}
The polymerase chain reaction (PCR) has made possible the rapid isolation and amplification of specific DNA segments, which can then be used for a wide range of applications (1). Direct sequencing of PCR products has been described, using assymetric PCR, which requires purification of the PCR product, or inclusion of 10% DMSO in the sequencing reaction (2, 3, 4). However, these methods of sequencing have depended on the production of a pure uncontaminated DNA fragment. Direct sequencing from… CONTINUE READING

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Vaudin.M., Kettle.S. and Jones.D.S.C

J. P. Schofield
Nudeic Adds Res • 1989

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