Direct signal transduction via functional interferon-αβ receptors in CD34+ hematopoietic stem cells

@article{GironMichel2002DirectST,
  title={Direct signal transduction via functional interferon-αβ receptors in CD34+ hematopoietic stem cells},
  author={Julien Giron-Michel and D. Weill and Gregory G Bailly and St{\'e}phanie Legras and P C Nardeux and Bruno Azzarone and M G Tovey and Pierre Eid},
  journal={Leukemia},
  year={2002},
  volume={16},
  pages={1135-1142}
}
Affinity purified, freshly isolated CD34+ progenitors were shown to express low levels of type I interferon (IFN) receptors (740 ± 60 binding sites/cell, Kd 0.7 ± 0.04 nM) determined by Scatchard's analysis using a radiolabelled, neutralizing, monoclonal antibody directed against the IFNAR1 chain of the human type I IFN receptor. Treatment of freshly isolated (day 0), highly purified (>95% pure) CD34+ cells with recombinant IFN-α resulted in rapid tyrosine phosphorylation and activation of… CONTINUE READING