Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.

@article{Coupland2012DirectSO,
  title={Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.},
  author={Paul G. Coupland and T. Chandra and Michael A. Quail and Wolf Reik and Harold Swerdlow},
  journal={BioTechniques},
  year={2012},
  volume={53 6},
  pages={365-72}
}
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1… CONTINUE READING
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