Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.

  title={Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.},
  author={Paul G. Coupland and T. Chandra and Michael A. Quail and Wolf Reik and Harold Swerdlow},
  volume={53 6},
We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1… CONTINUE READING
Highly Cited
This paper has 27 citations. REVIEW CITATIONS

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 21 extracted citations


Publications referenced by this paper.
Showing 1-10 of 19 references

Sensitive and specific single - molecule sequencing of 5hydroxymethylcytosine

  • I. A. Murray, T. A. Clark, +5 authors S. W. Turner
  • Nat . Methods
  • 2012

The evolution of plasmidcarried antibiotic resistance

  • E. Saras, V. Metayer, B. Doublet, A. Cloeckaert
  • BMC Evol . Biol .
  • 2012

Origins of the E . coli strain causing an outbreak of hemolyticuremic syndrome in Germany

  • C. S. Chin, D. R. Rank, J. S. Eid, S. W. Turner
  • N . Engl . J . Med .
  • 2010

Spread of the blaTEM52 gene is mainly ensured by IncI 1 / ST 36 plasmids in Escherichia coli isolated from cattle in France

  • E. Miró, C. Segura, +5 authors M. Salvadó
  • J Antimicrob Chemother .
  • 2010

The methylomes of six bacteria

  • M. T. Holden, J. A. Lindsay, +5 authors J. Parkhill
  • Nucleic Acids Res .
  • 2010

Similar Papers

Loading similar papers…