Direct selection of stabilised yeast URA3 transformants with 5- fluorouracil

Abstract

Yeast expression vectors are generally based on the 2 micron plasmid and are not stably inherited, so that continued selection pressure must be applied to prevent the gradual accumulation of plasmid-free cells. A number of 'autoselection' systems have been proposed and perhaps the most versatile is the stabilisation of plasmids containing the URA3 gene in… (More)
DOI: 10.1093/nar/19.1.187

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Cite this paper

@article{Romanos1991DirectSO, title={Direct selection of stabilised yeast URA3 transformants with 5- fluorouracil}, author={Michael A. Romanos and Katrina M. Beesley and Jeffrey J. Clare}, journal={Nucleic acids research}, year={1991}, volume={19 1}, pages={187} }