Direct selection of a specifically blocked mutant of Actinomadura brunnea. Isolation of a third 8-methoxy substituted chlortetracycline.

@article{Smith1987DirectSO,
  title={Direct selection of a specifically blocked mutant of Actinomadura brunnea. Isolation of a third 8-methoxy substituted chlortetracycline.},
  author={E. B. Smith and Hanan Munayyer and Matthew J. Ryan and B A Mayles and Vinod R. Hegde and George H. Miller},
  journal={The Journal of antibiotics},
  year={1987},
  volume={40 10},
  pages={
          1419-25
        }
}
Actinomadura brunnea produces 2'-N-methyl-8-methoxychlortetracycline. A derivative of this strain has been isolated that is specifically blocked in methylation of the 2'-amino position. This isolate was detected by screening approximately 30,000 colonies of a mutagenized population of Actinomadura brunnea using a direct soft agar overlay with an Escherichia coli indicator. The antibiotic produced by the blocked mutant has been identified as 8-methoxychlortetracycline (Sch 36969) based upon its… 
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