Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.

@article{Lovett1991DirectSA,
  title={Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.},
  author={Michael Lovett and Juha Kere and Lynene Hinton},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 21},
  pages={9628-32}
}
We have developed a strategy for the rapid enrichment and identification of cDNAs encoded by large genomic regions. The basis of this "direct selection" scheme is the hybridization of an entire library of cDNAs to an immobilized genomic clone. Nonspecific hybrids are eliminated and selected cDNAs are eluted. These molecules are then amplified and are either cloned or subjected to further selection/amplification cycles. This scheme was tested using a 550-kilobase yeast artificial chromosome… CONTINUE READING