Direct saliva transcript analysis as a novel non-invasive method for oestrus marker detection in buffaloes.

Abstract

Salivary RNA-based biomarkers are not available for any physiological condition in farm animals. Hence, an objective of this study was to perform salivary transcript analysis in buffaloes. Saliva, after removal of the cells and particulate matter, was directly used for RT-PCR without RNA isolation. Direct saliva transcript analysis (DSTA) showed a… (More)
DOI: 10.3109/1354750X.2015.1118549

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