Direct quantification of the enteric bacterium Oxalobacter formigenes in human fecal samples by quantitative competitive-template PCR.

@article{Sidhu1999DirectQO,
  title={Direct quantification of the enteric bacterium Oxalobacter formigenes in human fecal samples by quantitative competitive-template PCR.},
  author={Harmeet Sidhu and Ross Philip Holmes and Marvin J. Allison and Ammon Broughton Peck},
  journal={Journal of clinical microbiology},
  year={1999},
  volume={37 5},
  pages={1503-9}
}
Homeostasis of oxalic acid appears to be regulated, in part, by the gut-associated bacterium Oxalobacter formigenes. The loss of this bacterium from the gut flora is associated with an increased susceptibility to hyperoxaluria, a condition which can lead to the formation of calcium oxalate crystalluria and kidney stones. In order to identify and quantify the presence of O. formigenes in clinical specimens, a quantitative-PCR-based assay system utilizing a competitive DNA template as an internal… CONTINUE READING
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