Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides.

@article{Wefers2013DirectPO,
  title={Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides.},
  author={Benedikt Wefers and Melanie Meyer and Oskar Ortiz and Martin Hrabě de Angelis and Jens J\orgen Hansen and Wolfgang Wurst and Ralf K{\"u}hn},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 10},
  pages={3782-7}
}
The study of genetic disease mechanisms relies mostly on targeted mouse mutants that are derived from engineered embryonic stem (ES) cells. Nevertheless, the establishment of mutant ES cells is laborious and time-consuming, restricting the study of the increasing number of human disease mutations discovered by high-throughput genomic analysis. Here, we present an advanced approach for the production of mouse disease models by microinjection of transcription activator-like effector nucleases… CONTINUE READING
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