Direct observation of the participation of flavin in product formation by thyX-encoded thymidylate synthase.

@article{Gattis2005DirectOO,
  title={Direct observation of the participation of flavin in product formation by thyX-encoded thymidylate synthase.},
  author={Samuel G. Gattis and Bruce A Palfey},
  journal={Journal of the American Chemical Society},
  year={2005},
  volume={127 3},
  pages={
          832-3
        }
}
The synthesis of thymine for DNA is catalyzed by the enzyme thymidylate synthase (TS). A family of flavin-dependent TSs encoded by the thyX gene has been discovered recently. These newly discovered TSs require a reducing substrate in addition to 2'-deoxyuridine monophosphate (dUMP) and 5,10-methylenetetrahydrofolate (CH2THF), suggesting that the enzyme-bound flavin is a redox intermediary in catalysis. The oxidation of the reduced flavin of the TS from Campylobacter jejuni has been observed… CONTINUE READING
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