Direct observation of bacterial exopolysaccharides in dairy products using confocal scanning laser microscopy.

@article{Hassan2002DirectOO,
  title={Direct observation of bacterial exopolysaccharides in dairy products using confocal scanning laser microscopy.},
  author={Ashraf N Hassan and Joseph F. Frank and Karsten Bruun Qvist},
  journal={Journal of dairy science},
  year={2002},
  volume={85 7},
  pages={
          1705-8
        }
}
The objective of this work was to develop a methodology for direct visualization of bacterial exopolysaccharides (EPS) in fully hydrated dairy products. The new method involved staining EPS with wheat germ agglutinin labeled with Alexa fluor 488 or staining with concanavalin A 488. Samples were observed using confocal scanning laser microscopy. Distribution of EPS produced by Lactococcus lactis (CHCC 3367), a combination of Streptococcus thermophilus (CHCC 3534) and Lactobacillus delbrueckii… CONTINUE READING
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