Direct monitoring of albumin lysine-525 N-homocysteinylation in human serum by liquid chromatography/mass spectrometry.

@article{Sikora2010DirectMO,
  title={Direct monitoring of albumin lysine-525 N-homocysteinylation in human serum by liquid chromatography/mass spectrometry.},
  author={Marta Sikora and Lukasz Marczak and Tomasz Twardowski and Maciej Stobiecki and Hieronim Jakubowski},
  journal={Analytical biochemistry},
  year={2010},
  volume={405 1},
  pages={132-4}
}
A posttranslational protein modification by homocysteine-thiolactone (N-homocysteinylation) is linked to human vascular and neurodegenerative diseases. Although chemical and immunological methods are available to detect and quantify the extent of protein N-homocysteinylation, the determination of site-specific N-homocysteinylation in vivo remains challenging. Here we describe a liquid chromatography/mass spectrometry method that monitors the extent of N-homocysteinylation at albumin lysine-525… CONTINUE READING