Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells.

@article{Scott2013DirectME,
  title={Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells.},
  author={Daniel J Scott and Andreas Pl{\"u}ckthun},
  journal={Journal of molecular biology},
  year={2013},
  volume={425 3},
  pages={662-77}
}
G protein-coupled receptors (GPCRs) are the largest class of pharmaceutical protein targets, yet drug development is encumbered by a lack of information about their molecular structure and conformational dynamics. Most mechanistic and structural studies as well as in vitro drug screening with purified receptors require detergent solubilization of the GPCR, but typically, these proteins exhibit only low stability in detergent micelles. We have developed the first directed evolution method that… CONTINUE READING
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