Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.

@article{Fontana2006DirectMA,
  title={Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.},
  author={Cecilia Alejandra Fontana and Pier Sandro Cocconcelli and Graciela Margarita Vignolo},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 8},
  pages={5618-22}
}
Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8 degrees C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial… CONTINUE READING

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Observations on the succession dynamics of lactic acid bacteria populations in chill-stored vacuum-packaged beef

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