Direct measurement of the passive stiffness of rat sperm and implications to the mechanism of the calcium response.

@article{SchmitzLesich2004DirectMO,
  title={Direct measurement of the passive stiffness of rat sperm and implications to the mechanism of the calcium response.},
  author={Kathleen A Schmitz-Lesich and Charles B. Lindemann},
  journal={Cell motility and the cytoskeleton},
  year={2004},
  volume={59 3},
  pages={169-79}
}
Glass microprobes were used to measure the stiffness of the flagella of Triton X-100-extracted rat sperm models. The sperm models were treated with 50 microM sodium vanadate and 0.1 mM Mg-ATP to evaluate the stiffness of the passive flagellar structure without the influence of the dynein motor proteins. The passive stiffness was determined to be 4.6 (+/- 1.1) x 10(-19) N x m(2). Rat sperm models exposed to greater than 10(-5) M calcium ions exhibit a strong bend in the basal 40 microm of the… CONTINUE READING

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Structural-functional relationships of the dynein, spokes, and central-pair projections predicted from an analysis of the forces acting within a flagellum

  • Lindemann CB.
  • Biophys J 84:4115–4126.
  • 2003

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