Direct measurement of nucleation and growth rates in lysozyme folding.

@article{Kiefhaber1997DirectMO,
  title={Direct measurement of nucleation and growth rates in lysozyme folding.},
  author={Thomas Kiefhaber and Annett Bachmann and G Wildegger and Conrad Wagner},
  journal={Biochemistry},
  year={1997},
  volume={36 17},
  pages={5108-12}
}
A kinetic folding intermediate of hen lysozyme is shown to form in a nucleation/growth type of mechanism. Under native solvent conditions, a nucleated state is formed slowly during refolding (tau = 14 +/- 1 ms at 0 M GdmCl) and is rapidly converted to the folding intermediate (tau = 300 +/- 150 micros at 0 M GdmCl). Under these conditions the nucleated state represents a high-energy state compared to the folding intermediate (delta deltaG0 = 13.7 +/- 3 kJ/mol). At elevated concentrations of… CONTINUE READING