Direct measurement of hormone-induced acidification in intact bone.

  title={Direct measurement of hormone-induced acidification in intact bone.},
  author={Glenn Scott Belinsky and Armen H. Tashjian},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  volume={15 3},
Previous findings have shown that osteoblasts respond to parathyroid hormone (PTH) with an increase in extracellular acidification rate (ECAR) in addition to the known effect of PTH to increase local acidification by osteoclasts. We, therefore, investigated use of the Cytosensor to measure the ECAR response of whole intact bone to PTH employing microphysiometry. The Cytosensor measures a generic metabolic increase of cells to various agents. Using neonatal mouse calvaria, we found that the area… CONTINUE READING