Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium.

@article{Tjandra1997DirectMO,
  title={Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium.},
  author={Nico Tjandra and Adriaan Bax},
  journal={Science},
  year={1997},
  volume={278 5340},
  pages={
          1111-4
        }
}
In isotropic solution, internuclear dipolar couplings average to zero as a result of rotational diffusion. By dissolving macromolecules in a dilute aqueous nematic discotic liquid-crystalline medium containing widely spaced magnetically oriented particles, a tunable degree of solute alignment with the magnetic field can be created while retaining the high resolution and sensitivity of the regular isotropic nuclear magnetic resonance (NMR) spectrum. Dipolar couplings between 1H-1H, 1H-13C, 1H… 
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