Direct ligand–receptor complex interaction controls Brassica self-incompatibility

@article{Takayama2001DirectLC,
  title={Direct ligand–receptor complex interaction controls Brassica self-incompatibility},
  author={Seiji Takayama and Hiroko Shimosato and Hiroshi Shiba and Miyuki Funato and F S Che and Masao Watanabe and Megumi Iwano and Akira Isogai},
  journal={Nature},
  year={2001},
  volume={413},
  pages={534-538}
}
Many higher plants have evolved self-incompatibility mechanisms to prevent self-fertilization. In Brassica self-incompatibility, recognition between pollen and the stigma is controlled by the S locus, which contains three highly polymorphic genes: S-receptor kinase (SRK), S-locus protein 11 (SP11) (also called S-locus cysteine-rich protein; SCR) and S-locus glycoprotein (SLG). SRK encodes a membrane-spanning serine/threonine kinase that determines the S-haplotype specificity of the stigma, and… Expand
Molecular mechanism of self-recognition in Brassica self-incompatibility.
TLDR
Biochemical studies have revealed that SP11 functions as the sole ligand for its cognate SRK receptor complex, and this interaction induces the autophosphorylation of SRK, which is expected to trigger the signalling cascade that results in the rejection of self-pollen. Expand
Direct interaction between S-locus receptor kinase and M-locus protein kinase involved in Brassica self-incompatibility signaling
TLDR
Results suggest that MLPK could be a direct target of SRK in Brassica SI signaling, and an in vitro phosphorylation assay indicated that the kinase domain ofSRK could efficiently phosphorylate MLPK. Expand
Calcium signalling mediates self-incompatibility response in the Brassicaceae
TLDR
It is shown that Ca2+ influx into stigma papilla cells mediates self-incompatibility signalling, and this results suggest that Ca 2+ influx mediated by GLR is the essential self- incompatibility response leading to self-pollen rejection. Expand
Characterization of the SP11/SCR High-Affinity Binding Site Involved in Self/Nonself Recognition in Brassica Self-Incompatibility
TLDR
The 60-kD stigmatic membrane protein is a truncated form of SRK containing the extracellular domain, transmembrane domain, and part of the juxtamembrane Domain, which will be significant for the specific perception of SP11 by SRK. Expand
A conserved role for the ARC1 E3 ligase in Brassicaceae self-incompatibility
TLDR
A mini review highlights the recent progress in establishing ARC1’s conserved role in self-pollen rejection in Brassica and Arabidopsis species and discusses future research directions in this field. Expand
The Recognition and Rejection of self-incompatible Pollen in the Brassicaceae
TLDR
With the premise that pollen rejection occurs by blocking cellular responses in the stigma required for the acceptance of compatible pollen, Exo70A1 has been identified as a 'compatibility' factor that is negatively regulated by the self-incompatibility pathway. Expand
A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling
TLDR
Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling. Expand
S cysteine-rich (SCR) binding domain analysis of the Brassica self-incompatibility S-locus receptor kinase.
TLDR
It is shown that residues within the hypervariable subdomain define SRK binding and are likely to be involved in defining haplotype specificity. Expand
Structure of the Male Determinant Factor for Brassica Self-incompatibility*
TLDR
The solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype, is described, which folds into an α/β sandwich structure that resembles those of plant defensins. Expand
Functional test of Brassica self-incompatibility modifiers in Arabidopsis thaliana
TLDR
It is shown that the A. thaliana ARC1 ortholog is a highly decayed pseudogene, and that, unlike reports in Brassica, inactivation of the MLPK ortholog AtAPK1b and overexpression of Exo70A1 neither abolish nor weaken SI in A.Thaliana SRK-SCR plants. Expand
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TLDR
It is shown that by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG 28 transgene separately, that expression of SRK 28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants, and that the ability ofSRK28 to reject S28 pollen was enhanced bySLG 28. Expand
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TLDR
It is shown thatSRK is phosphorylated in vivo within one hour of self-pollination, and that, in vitro, autophosphorylation of SRK is prevented by the stigma thioredoxin THL1 in the absence of a ligand. Expand
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TLDR
The identified SP11 gene from three additional S haplotypes and further characterized the gene strongly suggest that SP11 is the pollen S determinant in SI. Expand
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TLDR
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TLDR
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TLDR
In situ localization of PCP-A1 transcripts revealed that they accumulate specifically in pollen at the late binucleate/trinucleate stage of development rather than in the tapetum, which previously was taken to be the principal source of the pollen coat. Expand
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TLDR
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TLDR
Loss- of-function and gain-of-function studies prove that the SCR gene product is necessary and sufficient for determining pollen self-incompatibility specificity, possibly by acting as a ligand for the stigmatic receptor. Expand
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TLDR
The sequences of the S-glycoproteins from three homozygotes of Brassica campestris show extensive homology and have cysteine-rich clusters and 6–7 oligosaccharide chains. Expand
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The pollen–stigma interaction of self-incompatibility in the mustard family Brassicaceae is controlled by the S locus. Nearly fifty allelic variants at this locus control the specificity of thisExpand
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