Direct isolation of genes encoded within a homogeneously staining region by chromosome microdissection.

@article{Su1994DirectIO,
  title={Direct isolation of genes encoded within a homogeneously staining region by chromosome microdissection.},
  author={Yan A. Su and Jeffrey M. Trent and Xin-yuan Guan and Paul S. Meltzer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 19},
  pages={9121-5}
}
Identification of genes involved in recurring chromosome rearrangements has provided significant insight into the molecular basis of malignancy. We describe here a strategy combining chromosome microdissection and hybrid selection for the direct isolation of chromosome region-specific genes. We modeled this strategy by using sequences recovered from the microdissection of a homogeneously staining region to allow isolation of genes that were overexpressed and present at high copy number within… CONTINUE READING