Direct isolation of Francisella spp. from environmental samples.

@article{Petersen2009DirectIO,
  title={Direct isolation of Francisella spp. from environmental samples.},
  author={Jeannine M. Petersen and John Carlson and Brook M. Yockey and Satish Pillai and Cheryl Kuske and G Garbalena and Sudha Pottumarthy and Linda J Chalcraft},
  journal={Letters in applied microbiology},
  year={2009},
  volume={48 6},
  pages={663-7}
}
AIMS To develop a selective medium for isolation of F. tularensis, F. novicida and F. philomiragia from environmental samples. METHODS AND RESULTS A selective media, cysteine heart agar with 9% chocolatized sheep blood, containing polymyxin B, amphotericin B, cyclohexamide, cefepime and vancomycin (CHAB-PACCV) was developed and evaluated for growth of Francisella spp. No differences were observed in recovered colony forming units (CFUs) for F. tularensis, F. novicida and F. philomiragia on… CONTINUE READING

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