Direct interactions among Ret, GDNF and GFRalpha1 molecules reveal new insights into the assembly of a functional three-protein complex.

@article{Amoresano2005DirectIA,
  title={Direct interactions among Ret, GDNF and GFRalpha1 molecules reveal new insights into the assembly of a functional three-protein complex.},
  author={Angela Amoresano and Mariarosaria Incoronato and Gianluca Monti and Piero Pucci and Vittorio de Franciscis and Laura Cerchia},
  journal={Cellular signalling},
  year={2005},
  volume={17 6},
  pages={717-27}
}
The glial-cell-line-derived neurotrophic factor (GDNF) ligand activates the Ret receptor through the assembly of a multiprotein complex, including the GDNF family receptor alpha1 (GFRalpha1) molecule. Given the neuroprotective role of GDNF, there is an obvious need to precisely identify the structural regions engaged in direct interactions between the three molecules. Here, we combined a functional approach for Ret activity (in PC12 cells) to cross-linking experiments followed by MS-MALDI to… CONTINUE READING

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