Direct interaction of a Ca2+-binding loop of synaptotagmin with lipid bilayers.

  title={Direct interaction of a Ca2+-binding loop of synaptotagmin with lipid bilayers.},
  author={Edwin R Chapman and Anson F Davis},
  journal={The Journal of biological chemistry},
  volume={273 22},
Synaptotagmin 1 binds Ca2+ and membranes via its C2A-domain and plays an essential role in excitation-secretion coupling. In this study, we sought to identify Ca2+- and membrane-induced local conformational changes in the C2A-domain of synaptotagmin and to delineate the C2A-lipid binding interface. To address these questions native phenylalanine residues were replaced, at each face of the domain, with tryptophan reporters. Changes in tryptophanyl fluorescence indicated that Ca2+ induced long… CONTINUE READING


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