Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework.

@article{Maur2002DirectIV,
  title={Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework.},
  author={Adrian Auf der Maur and Christian Zahnd and Franziska Fischer and Silvia Spinelli and Annemarie Honegger and Christian Cambillau and Dominik Escher and Andreas Pl{\"u}ckthun and Alcide Barberis},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 47},
  pages={45075-85}
}
Single-chain Fv antibody fragments (scFv) represent a convenient antibody format for intracellular expression in eukaryotic or prokaryotic cells. These so-called intrabodies have great potential in functional genomics as a tool to study the function of newly identified proteins in vivo, for example by binding-induced modulation of their activity or by blocking interactions with other proteins. However, the intracellular expression and activity of many single-chain Fvs are limited by their… CONTINUE READING