Direct in vitro selection of a 2'-O-methyl aptamer to VEGF.

@article{Burmeister2005DirectIV,
  title={Direct in vitro selection of a 2'-O-methyl aptamer to VEGF.},
  author={Paula E Burmeister and Scott D. Lewis and Robert F. Silva and Jeffrey R Preiss and Lillian R Horwitz and Patrick Shannon Pendergrast and Thomas G. McCauley and Jeffrey C. Kurz and David M. Epstein and Charles C. Wilson and Anthony D. Keefe},
  journal={Chemistry & biology},
  year={2005},
  volume={12 1},
  pages={25-33}
}
Aptamers (protein binding oligonucleotides) have potential as a new class of targeted therapeutics. For applications requiring chronic systemic administration, aptamers must achieve high-affinity target binding while simultaneously retaining high in vivo stability, tolerability, and ease of chemical synthesis. To this end, we describe a method for generating aptamers composed entirely of 2'-O-methyl nucleotides (mRmY). We present conditions under which 2'-O-methyl transcripts can be generated… CONTINUE READING

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