Direct in vitro selection of a 2'-O-methyl aptamer to VEGF.

  title={Direct in vitro selection of a 2'-O-methyl aptamer to VEGF.},
  author={Paula E Burmeister and Scott D. Lewis and Robert F. Silva and Jeffrey R Preiss and Lillian R Horwitz and Patrick Shannon Pendergrast and Thomas G. McCauley and Jeffrey C. Kurz and David M. Epstein and Charles C. Wilson and Anthony D. Keefe},
  journal={Chemistry & biology},
  volume={12 1},
Aptamers (protein binding oligonucleotides) have potential as a new class of targeted therapeutics. For applications requiring chronic systemic administration, aptamers must achieve high-affinity target binding while simultaneously retaining high in vivo stability, tolerability, and ease of chemical synthesis. To this end, we describe a method for generating aptamers composed entirely of 2'-O-methyl nucleotides (mRmY). We present conditions under which 2'-O-methyl transcripts can be generated… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 96 extracted citations

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

International journal of molecular sciences • 2017
View 9 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…