Direct identification of mycobacteria from clinical specimens by multiplex real-time PCR.

Abstract

AIMS To directly identify clinically relevant mycobacteria from clinical specimens, we have developed a multiplex real-time PCR assay with hydrolysis probes that can identify 20 mycobacterial species. METHODS AND RESULTS The assay was initially evaluated using 248 strains, including both reference strains and clinical isolates. Then, the assay was… (More)
DOI: 10.1111/jam.12780

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