Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene: evaluation of BACTEC 9240 instrument true-positive and false-positive results.

@article{Qian2001DirectIO,
  title={Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene: evaluation of BACTEC 9240 instrument true-positive and false-positive results.},
  author={Qi Qian and Yi-Wei Tang and Christopher P. Kolbert and Colleen Torgerson and John G. Hughes and Emily A. Vetter and William Scott Harmsen and Stacy O. Montgomery and Franklin R. Cockerill and David H. Persing},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 10},
  pages={
          3578-82
        }
}
In a previous study which evaluated the BACTEC 9240 automated blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.), we noted a 1.3% "instrument false-positive" rate. That is, the BACTEC system signaled that a bottle (BACTEC Plus Aerobic/F bottle or BACTEC Anaerobic Lytic/10 bottle) culture was positive but a Gram stain was negative and there was no growth of bacteria or yeasts on subculture to chocolate agar. Furthermore, from the same sample of blood, cultures for… CONTINUE READING
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