Direct fluorochrome labeling of phage display library clones for studying binding specificities: applications in flow cytometry and fluorescence microscopy.

@article{Jaye2004DirectFL,
  title={Direct fluorochrome labeling of phage display library clones for studying binding specificities: applications in flow cytometry and fluorescence microscopy.},
  author={David L Jaye and Cissy M. Geigerman and Ross E Fuller and A. Levine Ian F. Akyildiz and Charles A Parkos},
  journal={Journal of immunological methods},
  year={2004},
  volume={295 1-2},
  pages={119-27}
}
Phage display technology is increasingly employed to identify high-affinity peptides and single-chain antibodies with binding specificities for a diversity of target types. The analysis of phage-binding sensitivity and specificity typically employs directly labeled secondary antiphage antibodies and potentially tertiary labels, such as fluorochromes and enzymes, when biotinylated antibodies are used. However, secondary or tertiary reagents may not be feasible or desirable for some target types… CONTINUE READING

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