Direct exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria.

Abstract

Microbial consortia that cooperatively exchange electrons play a key role in the anaerobic processing of organic matter. Interspecies hydrogen transfer is a well-documented strategy for electron exchange in dispersed laboratory cultures, but cooperative partners in natural environments often form multispecies aggregates. We found that laboratory evolution of a coculture of Geobacter metallireducens and Geobacter sulfurreducens metabolizing ethanol favored the formation of aggregates that were electrically conductive. Sequencing aggregate DNA revealed selection for a mutation that enhances the production of a c-type cytochrome involved in extracellular electron transfer and accelerates the formation of aggregates. Aggregate formation was also much faster in mutants that were deficient in interspecies hydrogen transfer, further suggesting direct interspecies electron transfer.

DOI: 10.1126/science.1196526

3 Figures and Tables

01002003002011201220132014201520162017
Citations per Year

1,244 Citations

Semantic Scholar estimates that this publication has 1,244 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Summers2010DirectEO, title={Direct exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria.}, author={Zarath M. Summers and Heather E. Fogarty and Ching Leang and Ashley E. Franks and Nikhil S. Malvankar and Derek R. Lovley}, journal={Science}, year={2010}, volume={330 6009}, pages={1413-5} }