Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein.

Abstract

The BCR-ABL oncogene is central in the pathogenesis of chronic myeloid leukemia (CML). Here, tandem nanospray mass spectrometry was used to demonstrate cell surface HLA-associated expression of the BCR-ABL peptide KQSSKALQR on class I-negative CML cells transfected with HLA-A*0301, and on primary CML cells from HLA-A3-positive patients. These patients mounted a cytotoxic T-lymphocyte response to KQSSKALQR that also killed autologous CML cells, and tetramer staining demonstrated the presence of circulating KQSSKALQR-specific T cells. The findings are the first demonstration that CML cells express HLA-associated leukemia-specific immunogenic peptides and provide a sound basis for immunization studies against BCR-ABL.

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@article{Clark2001DirectET, title={Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein.}, author={R. E. Clark and I. Anthony Dodi and Sue Hill and Jennie R Lill and Gr{\'e}gory Aubert and Andrew R. Macintyre and Jos{\'e} Arturo Marcial Rojas and Alice Bourdon and Philip L R Bonner and L. Wang and Stephen E Christmas and Paul Travers and Colin S Creaser and Robert C. Rees and Jos{\`e} Alejandro Madrigal}, journal={Blood}, year={2001}, volume={98 10}, pages={2887-93} }