Direct evidence for a primary immune response of murine B-lymphocytes after in vitro immunization of dissociated splenocytes.

Abstract

Monoclonal antibodies against human thyroglobulin were generated using splenocytes cultured in vitro with the antigen. When splenocytes from non-immunized mice were used, about 90% of the hybridomas obtained produced immunoglobulins of the IgM class. In contrast, when splenocytes from mice previously immunized in vivo with human thyroglobulin were cultured… (More)

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@article{Boer1987DirectEF, title={Direct evidence for a primary immune response of murine B-lymphocytes after in vitro immunization of dissociated splenocytes.}, author={Menko-Jan de Boer and Ferry A Ossendorp and Peter F. Bruning and Joseph M. Tager}, journal={Hybridoma}, year={1987}, volume={6 3}, pages={253-8} }