Direct evidence for GTP and GDP-Pi intermediates in microtubule assembly.

  title={Direct evidence for GTP and GDP-Pi intermediates in microtubule assembly.},
  author={Ronald Melki and M F Carlier and Dominique Pantaloni},
  volume={29 38},
Identification of the kinetic intermediates in GTP hydrolysis on microtubules and characterization of their assembly properties is essential in understanding microtubule dynamics. By using an improved glass filter assay that selectively traps microtubules with a dead time of 2 s and monitoring taxol-induced rapid assembly of microtubules from [gamma-32P,3H]GTP-tubulin 1:1 complex, direct evidence has been obtained for GTP- and GDP-Pi-microtubule transient states in the early stages of the… CONTINUE READING


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Microtubule polymerization dynamics.

Annual review of cell and developmental biology • 1997
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