Direct estimate of active bacteria: CTC use and limitations.

  title={Direct estimate of active bacteria: CTC use and limitations.},
  author={V{\'e}ronique Cr{\'e}ach and Anne Claire Baudoux and Georges Bertru and Bertrand Le Rouzic},
  journal={Journal of microbiological methods},
  volume={52 1},
During the last 10 years, the dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) has been used to determine the in situ number of "active" bacteria in different ecosystems. A part of this success is due to a simple protocol, which does not require sophisticated equipment. However, it has not been established whether the method determines viable cells, e.g. those capable of growth and cell division, as opposed to cells that are active in the sense of having some detectable metabolic activity. In… CONTINUE READING