Direct electrochemistry of immobilized human cytochrome P450 2E1.

  title={Direct electrochemistry of immobilized human cytochrome P450 2E1.},
  author={Andrea Fantuzzi and Michael Fairhead and Gianfranco Gilardi},
  journal={Journal of the American Chemical Society},
  volume={126 16},
This communication reports the first electrochemical study of the human P450 2E1 either absorbed or covalently linked to different electrode surfaces. Glassy-carbon and gold electrodes gave reversible electrochemical signals of an active P450 2E1. Molecular modeling of the enzyme helped to rationalize the results. A monolayer coverage was obtained on gold modified with cystamine/maleimide that covalently linked surface accessible cysteines of P450 2E1. The midpoint potential measured for the… CONTINUE READING