Direct electrochemical genosensing for multiple point mutation detection of Mycobacterium tuberculosis during the development of rifampin resistance.

@article{Kara2009DirectEG,
  title={Direct electrochemical genosensing for multiple point mutation detection of Mycobacterium tuberculosis during the development of rifampin resistance.},
  author={Pinar Gizem Kara and Cengiz Çavuşoğlu and Seda Çavdar and Mehmet Ozsoz},
  journal={Biosensors & bioelectronics},
  year={2009},
  volume={24 6},
  pages={
          1796-800
        }
}
We present a robust and simple method for the direct detection of multiple point mutations in the Mycobacterium tuberculosis rpoB gene during the development of rifampin (RIF) resistance using an electrochemical genosensor. The device contained five different capture probes which are designed to hybridize with several sequence segments within the bacterial rpoB gene hotspot region. Point mutations were detected by monitoring the guanine oxidation with differential pulse voltammetry after… CONTINUE READING
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