Direct effects of phorbol esters and diacylglycerols on the T-tubule Mg(2+)-ATPase.

@article{Kang1991DirectEO,
  title={Direct effects of phorbol esters and diacylglycerols on the T-tubule Mg(2+)-ATPase.},
  author={Jisun Kang and Henri Cunningham and Christine Jachec and A. G. Priest and A. Stephen Dahms and Roger Allen Sabbadini},
  journal={Archives of biochemistry and biophysics},
  year={1991},
  volume={290 1},
  pages={214-23}
}
T-tubule membrane vesicles isolated from skeletal muscle contain a very active Mg(2+)-ATPase (EC 3.6.1.34) which is modulated by lectins and is located in the junctional region near the sarcoplasmic reticulum membranes (1). The effects of several prominent lipophilic agents upon the ATPase have led us to evaluate the action of diacylglycerols and phorbol esters upon the enzyme. The ATPase is inhibited by submicromolar levels of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and… CONTINUE READING