Corpus ID: 24374038

Direct effect of metolazone on sodium-dependent transport across the renal brush border membrane.

  title={Direct effect of metolazone on sodium-dependent transport across the renal brush border membrane.},
  author={Stephen A. Kempson and Joseph C. Kowalski and Jules B. Puschett},
  journal={The Journal of laboratory and clinical medicine},
  volume={101 2},
Studies with clearance and micropuncture techniques indicate that metolazone inhibits transport of sodium and phosphate in the proximal tubule. The present study is focused on transport across the luminal BBM of the proximal tubule to determine whether metolazone has any direct effect on this initial step in transtubular reabsorption. Addition of metolazone (0.01 to 1.00 mM) to isolated renal BBM vesicles caused dose-dependent inhibition (30% to 70%) of the initial uphill phase of Na+ gradient… Expand
9 Citations
Diuretic effects on renal brush border membrane transport and metabolism.
It is concluded that metolazone inhibits phosphate transport through an effect on the BBM and does not affect renal gluconeogenesis in the rat. Expand
Inhibitory effects of volume expansion performed in vivo on transport in the isolated rabbit proximal tubule perfused in vitro.
Findings indicate that the inhibition of renal ionic transport by VE occurs in both PCT and PST and is, in part, the result of a direct effect of VE on tubular transport mechanisms. Expand
Inhibition by volume expansion of phosphate uptake by the renal proximal tubule brush border membrane.
The data indicate that the phosphaturia which resulted from the expansion procedure was accompanied by an inhibition of proximal BBM phosphate uptake, compatible with the view that the membrane transport changes resulted from alterations induced by the saline loading itself, rather than (or in addition to) any changes caused by parathyroid hormone excretion. Expand
Brush border membrane phosphate transport effects of volume expansion.
Volume expansion reproducibly results in the development of phosphaturia both in the experimental animal and in man, and when the parathyroid glands are removed however, phosphate transport in later nephron segments appears to be enhanced such that volume expansion is less phosphaturic inParathyroidectomized than in intact animals. Expand
Diuretics. Clinical pharmacology and therapeutic use (Part I).
The haemodynamic, humoral and physical factors involved in control of electrolyte and fluid handling by the kidney in normal conditions and pathological states are discussed in relation to rational choices of different diuretics in the treatment of various oedematous and non-oedEMatous conditions. Expand
Diuretics. Clinical pharmacology and therapeutic use (Part II).
The metabolic consequences of continued diuretic usage are considered along with non-metabolic sequelae such as ototoxicity or interactions with other concurrent treatments, and the relationships between the clinical benefits conferred and the potential harms generated by long term diuretics are discussed. Expand
Diuretic Combinations in Refractory Oedema States
The rationale for and use of various diuretic combinations, with particular emphasis on the metolazone-loop diUREtic combination, is reviewed here and applied to the major disease states associated with diuretics resistance. Expand
Metolazone and its role in edema management.
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If an excessive diuresis occurs with a metolazone and loop diuretic combination both drugs should be stopped temporarily and the temptation should be avoided to simply reduce the doses of either metolAZone or the loop diUREtic as a means to controlling an active diureis. Expand
Pharmacology of recombinant human GABAA receptor subtypes measured using a novel pH-based high-throughput functional efficacy assay
A novel fluorescence-based high throughput functional assay allows the rapid characterization of allosteric ligands acting on human GABA(A) receptors, which indicates that the alpha subunit within the recombinant receptor expressed in L(tk-) cells can affect the efficacy of the response to some BZ compounds. Expand