Direct effect of insulin and insulin-like growth factor-I on the secretory activity of rat pancreatic beta cells

@article{Schravendijk1990DirectEO,
  title={Direct effect of insulin and insulin-like growth factor-I on the secretory activity of rat pancreatic beta cells},
  author={C. F. H. Van Schravendijk and Lieven Heylen and J. L. van den Brande and Daniel G. Pipeleers},
  journal={Diabetologia},
  year={1990},
  volume={33},
  pages={649-653}
}
Purified pancreatic Beta cells were labelled with 3H-tyrosine before studying their secretory activity in perifusion. At 1.4 mmol/l glucose, the cells released similar fractions (0.01% per min) of their contents in preformed and in newly formed insulin. At 20 mmol/l glucose plus 10−8 mol/l glucagon, these fractional release rates increased by 16 and 40-fold respectively. The preferential release of newly synthesized as compared to stored insulin is attributable to a heterogeneity in individual… CONTINUE READING