Direct effect of electrical stimulation on induction of brain-derived neurotrophic factor from cultured retinal Müller cells.

@article{Sato2008DirectEO,
  title={Direct effect of electrical stimulation on induction of brain-derived neurotrophic factor from cultured retinal M{\"u}ller cells.},
  author={Tatsuhiko Sato and Takashi Fujikado and T Lee and Yasuo Tano},
  journal={Investigative ophthalmology & visual science},
  year={2008},
  volume={49 10},
  pages={4641-6}
}
PURPOSE To investigate the direct effect of electrical stimulation (ES) on the induction of brain-derived neurotrophic factor (BDNF) from cultured retinal Müller cells. METHODS Müller cells were isolated from rat retinas. ES was applied to passage 1 Müller cells with biphasic pulses (duration, 1 ms; frequency, 20 Hz; current, 10 mA) for 30 minutes. The changes in gene expression after ES were analyzed with microarrays. The mRNA and protein levels of BDNF were determined at each time point… CONTINUE READING

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