Direct determination of tamoxifen and its four major metabolites in plasma using coupled column high-performance liquid chromatography.

Abstract

A rapid, rugged and fully automated method has been developed for the determination of tamoxifen and its major metabolites in plasma. The system is based upon an in-line extraction process combined with column switching to a coupled analytical column. The plasma sample is deproteinated by the addition of acetonitrile before injection onto a semi-permeable surface (SPS) cyano guard column (1.0 x 0.46 cm I.D.). After washing the guard column briefly with water, the sample is eluted with a mobile phase composed of 35% acetonitrile in 20 mM potassium phosphate buffer (pH 3). The eluent is directed through a cyano analytical column (25 x 0.46 cm I.D.) and a photochemical reactor where the analytes are converted to highly fluorescent phenanthrene derivatives. Tamoxifen, 4-hydroxytamoxifen, N-desdimethyltamoxifen, N-desmethyltamoxifen and tamoxifen-ol are eluted in that order at a flow-rate of 1.0 ml/min. The method has been validated for use in a clinical study utilizing tamoxifen in the treatment of recurrent cerebral astrocytomas.

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@article{Fried1994DirectDO, title={Direct determination of tamoxifen and its four major metabolites in plasma using coupled column high-performance liquid chromatography.}, author={Karen Mandzak Fried and Irving W Wainer}, journal={Journal of chromatography. B, Biomedical applications}, year={1994}, volume={655 2}, pages={261-8} }