Direct determination of polyamines in human serum by radioimmunoassay.

  title={Direct determination of polyamines in human serum by radioimmunoassay.},
  author={Dagmar Bartos and Robert Hugh Campbell and Frantisek Bartos and D. P. Grettie},
  journal={Cancer research},
  volume={35 8},
Antipolyamine antibodies were produced following immunization of New Zealand white rabbits with thyroglobulin spermine conjugate, using multiple-site injection and macrophage-harvesting techniques. The antispermine antibody in a radioimmunoassay system cross-reacted with spermidine (22%), putrescine (1%), diaminopropane (6%), and cadaverine (0.16%). No reaction with L-lysine, ornithine, or histamine was noted. Using this antibody, the radioimmunoassay for direct determination of serum… CONTINUE READING


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