Direct determination of plasma fibrinogen levels by heat precipitation. A comparison of the technique against thrombin clottable fibrinogen with spectrophotometry and radial immuno-diffusion.

@article{Desvignes1981DirectDO,
  title={Direct determination of plasma fibrinogen levels by heat precipitation. A comparison of the technique against thrombin clottable fibrinogen with spectrophotometry and radial immuno-diffusion.},
  author={P Desvignes and Pierre Bonnet},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1981},
  volume={110 1},
  pages={9-17}
}
We have studied the Thorp method for precipitation of fibrinogen from diluted plasma, using a specific buffer (pH 6.3) at 56 degrees C, and the preferred anticoagulant (EDTA). Nephelometric or turbidimetric measurements of the precipitate were compared with the results obtained by radial immunodiffusion, or by thrombin clotting, of fibrinogen. In the absence of prior proteolysis (as indicated by the presence of fibrinogen and fibrin degradation products), correlations between the methods were… CONTINUE READING