Direct detection of small RNAs using splinted ligation

  title={Direct detection of small RNAs using splinted ligation},
  author={Patricia A. Maroney and Sangpen Chamnongpol and Fr{\'e}d{\'e}ric F. Souret and Timothy W Nilsen},
  journal={Nature Protocols},
This protocol describes a method for direct labeling and detection of small RNAs present in total RNA by splinted ligation. The assay uses a small RNA-specific bridge oligonucleotide to form base pairs with the small RNA and a 5′-end-radiolabeled ligation oligonucleotide. The captured small RNA is directly labeled by ligation. Detection of the labeled small RNAs is performed by denaturing gel electrophoresis and autoradiography or phosphorimaging. This protocol has been successfully used to… CONTINUE READING
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