Direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-PCR assay.

@article{Osiowy1998DirectDO,
  title={Direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-PCR assay.},
  author={Carla Osiowy},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 11},
  pages={
          3149-54
        }
}
  • Carla Osiowy
  • Published 1998 in Journal of clinical microbiology
Diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. To permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-PCR (RT-PCR) assay was developed. The assay included five primer sets that amplified the RNA of respiratory syncytial virus subtypes A and B, parainfluenza virus types 1, 2… CONTINUE READING
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