Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method.

@article{Ihira2007DirectDO,
  title={Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method.},
  author={Masaru Ihira and Shiho Akimoto and Fumi Miyake and Ayano Fujita and Ken Sugata and Sadao Suga and Masahiro Ohashi and Naoko Nishimura and Takao Ozaki and Yoshizo Asano and Tetsushi Yoshikawa},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2007},
  volume={39 1},
  pages={22-6}
}
BACKGROUND A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories. OBJECTIVES We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera. RESULTS The sensitivity of the HHV-6 LAMP protocol without heat… CONTINUE READING