Direct detection of activated platelets and platelet-derived microparticles in humans.

@article{Abrams1990DirectDO,
  title={Direct detection of activated platelets and platelet-derived microparticles in humans.},
  author={Charles S. Abrams and Norig Ellison and Andrei Z. Budzynski and Sanford J Shattil},
  journal={Blood},
  year={1990},
  volume={75 1},
  pages={128-38}
}
Flow cytometry was used to determine whether activated platelets and platelet-derived microparticles can be detected directly in whole blood after a hemostatic insult. Two different in vivo models of platelet activation were examined: (1) a standardized bleeding time, and (2) cardiopulmonary bypass. Platelets and microplatelets were identified with a biotinylated anti-glycoprotein (GP)lb antibody and a fluorophore, phycoerythrin-streptavidin. Microparticles were distinguished from platelets by… CONTINUE READING