Direct detection of DNA conformation in hybridization processes.

  title={Direct detection of DNA conformation in hybridization processes.},
  author={George Papadakis and Achilleas Tsortos and Florian Bender and Elena E Ferapontova and Electra Gizeli},
  journal={Analytical chemistry},
  volume={84 4},
DNA hybridization studies at surfaces normally rely on the detection of mass changes as a result of the addition of the complementary strand. In this work we propose a mass-independent sensing principle based on the quantitative monitoring of the conformation of the immobilized single-strand probe and of the final hybridized product. This is demonstrated by using a label-free acoustic technique, the quartz crystal microbalance (QCM-D), and oligonucleotides of specific sequences which, upon… CONTINUE READING