Direct demonstration of aquaporin-2 water channel recycling in stably transfected LLC-PK1 epithelial cells.

@article{Katsura1996DirectDO,
  title={Direct demonstration of aquaporin-2 water channel recycling in stably transfected LLC-PK1 epithelial cells.},
  author={Toshiya Katsura and Dennis A. Ausiello and Dennis L. Brown},
  journal={The American journal of physiology},
  year={1996},
  volume={270 3 Pt 2},
  pages={F548-53}
}
Vasopressin-dependent membrane insertion of aquaporin-2 (AQP-2) in collecting duct principal cells has been demonstrated in vivo and in vitro. However, the hypothesis that the AQP-2 molecule recycles between intracellular vesicles and the plasma membrane in response to hormonal stimulation and withdrawal remains to be demonstrated directly. In the present study, we examined AQP-2 recycling between intracellular vesicles and the plasma membrane in the absence of de novo protein synthesis using… CONTINUE READING

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