Direct delivery of exogenous MHC class I molecule-binding oligopeptides to the endoplasmic reticulum of viable cells.

@article{Day1997DirectDO,
  title={Direct delivery of exogenous MHC class I molecule-binding oligopeptides to the endoplasmic reticulum of viable cells.},
  author={Patricia M Day and Jonathan W. Yewdell and Angel Porgador and Ronald N. Germain and Jack R. Bennink},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 15},
  pages={
          8064-9
        }
}
After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER) by microscopy or by binding to radiolabeled class I molecules. ER delivery of a nonfluorescent peptide was demonstrated using a mAb highly specific for the peptide-class I molecule complex. ER localization of peptides: (i) required expression of appropriate class I molecules in the ER but not on the… CONTINUE READING

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Day et al

  • A. Porgador, Germain, 6 R.N.Immunity, 715–726. Immunology
  • Proc. Natl. Acad. Sci. USA 94
  • 1997
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